Trade.Information

Product Name: HEScGRO Medium for Human ES Cell Culture

Product Description

type: cell culture
catalog #: SCM020
UK Price: £ 176.00
pack size: 500 mL
                5 x 100 mL Aliquots

Description:
HEScGRO hES cell medium is the first, animal-component free medium that is specially formulated to meet the unique requirements of human embryonic stem cell culture. No other hES cell media is available as a serum-free, ready-to-use, and complete formulation. HEScGRO has been extensively tested and proven to maintain the pluripotential nature of several hES cell lines including: MEL-1, MEL-2 and H1. This medium is fully-defined, serum-free, and animal-component free, and does not require additional supplementation to maintain cells in their pluripotent state. Mitotically inactive feeder cell layers are commonly used to support hES cell growth. PLEASE NOTE: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.

Quality Assurance:
Each lot of medium is tested for the optimal cell growth and proliferation of MEL-1 Human ES cells
Sterility Testing: Negative
Osmolarity: 260-270 mOsm
pH: 7.2-7.3

Materials Required But Not Supplied:
Tissue Culture plates
Matrigel, 0.1 percent gelatin solution, human collagen IV or other tissue culture plastic coating material.
Mitocally inactivated Detroit 551 feeder cells are recommended (ATCC cat no. CCL-11) plated at 80,000 cells/cm2
Other feeder cells such as HS27 or WS1 may also be used, typically plated at 55,000 cell/cm2 IMPORTANT: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.

Formulation:
HEScGRO Medium is a proprietary, patent-pending formulation. The media formulation does not contain any animal components. It comes as a complete, ready-to-use formulation with (20 ng/mL) bFGF included.

Storage/Handling:
HEScGRO Serum-Free Medium should be stored at -20°C until ready to use. Upon thawing, the medium should be stored at 2-8°C and given a 2 week expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.

Tested Applications: Cell Culture

Species Reactivity: Human. Reactivity with other species has not been confirmed.

To Order, please
Call: 0800 0190 333
Fax: 0800 0190 444
Email: uk@chemicon.com

Company Details

6 September 2006
Stem Cell Sciences is awarded Small/Mid-Size Company Deal of the Year by Scottish Business Insider Deals & Dealmakers for the floatation of SCS plc on the London Stock Exchange Alternative Investment Market (AIM:... more