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Product Name: Fluorescence Flow Injection Analysis System
Product Description
The FIA-PMT-FL is a highly sensitive -- to parts per trillion --
photomultiplier-based flow through detection system ideally suited for ultra-low
fluorescence, chemiluminescence or bioluminescence measurements. The system's
heavy-duty, chemically resistant housing is built to withstand industrial
environments.
The FIA-PMT-FL has flow-through capability, but also accepts standard 1-cm path
length cuvettes for manual measurements. The FIA-PMT-FL is $6,495.
System Configuration
The FIA-PMT is built to order with either an internal excitation lamp or with an
optical fiber interfacing to an external lamp. Excitation lamp source options
include tungsten, mercury vapor, LEDs and laser diodes. Emission and excitation
filters are mounted in accessible slots inside the enclosure, allowing for easy
removal or exchange. The system uses an internal 25 mm flow cell.
Excitation Source Options
|
Internal excitation source options: |
Laser diodes (532 nm, 635 nm, 650 nm) |
|
Quartz tungsten lamp (440-750 nm) |
|
UV LED lamp (365-410 nm) |
|
Various Visible LED lamps |
|
Fiber optic excitation source options: |
Deuterium lamp (200-400 nm) |
|
Laser diodes (532 nm, 635 nm, 650 nm) |
|
Quartz tungsten lamp (440-750 nm) |
System Operation
The FIA-PMT-FL plugs into the RS-232 serial port of a personal computer and
comes with Windows-based software that allows the user to set the integration
time and voltage counts, and to obtain time histories of the measurements, both
plotted and tabulated. Also, the software can automatically create calibration
curves, and control a host of additional devices including various syringe and
peristaltic pumps, injection valves, selection valves, and autosamplers (please
inquire for details). For users who desire to control the PMT-FL from their own
software project, included is an ActiveX control for use with Visual Basic,
VC++, LabvVew, or nearly any other ActiveX- compatible development environment.
Typical Applications
Fluorometric ammonium analysis. Use the FIA-PMT-FL for the orthophtaldialdehyde
(OPA) method for NH4 assays, to ppb sensitivity. Results achieved with 365 nm
LED excitation source with broadband 410-490 nm emission filter.
Fluorescein-based assays. Use the FIA-PMT-FL to measure to ppb sensitivity.
Results achieved with Blue LED excitation source and broadband 510-590 nm
emission filter.
Additional Options
- Heated internal flow cell -- ambient to 60 °C, +/- 0.5 °C accuracy
- Dual-head PMT -- measure two excitation wavelength bands simultaneously
Specifications
| Dimensions: |
~13 cm x 18
cm x 25.4 cm |
| Spectral
range: |
310-750 nm
(filter/lamp selection dependent) |
| Detector: |
Photo-counting photomultiplier tube |
| PMT dynamic
range: |
2 x 10^6 |
| Responsivity: |
5 x 10^17
cps/watt (@ 400 nm) |
| Detection
limits: |
10 parts per
trillion measured with fluorescein @ 200 msec integration time and internal
LED |
| Pulse-pair
resolution: |
10 ns |
| Flow cell: |
10 mm path,
25 µL volume with flow-through cuvette |
| Filters: |
Your waveband
choice of 1 excitation filter and 1 emission filter (call for options) |
| Computer
interface: |
RS-232 to
Windows 98/Me/2000/XP OS |
Company Details
Ocean Optics is a leading supplier of solutions for optical sensing – fundamental methods of measuring and interpreting the interaction of light with matter. We enable diverse applications in medical and biological research, environmental... more
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| Suitable For: |
light - spectrometer - spectrometers - Ocean Optics - sampling accessories - collimating lens - cuvette holder - cuvette - sampling systems for fluid analysis - fluorescence sampling tools - light collection tools - light measurement - flow cells - fluorovette - positive displacement pump - optical post mounts - reflection measurement tools - metrology - filter holder - mapping table - integrating sphere - reflectance standard - nanopositiioner - specular reflectance - absorbance standard - NIST - Raman standard - probe - cosine correction - radiometric standard -
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