The new 1.7 mm triple- resonance Micro-CryoProbe has a total volume of 30
microliters and offers an increase in mass sensitivity of more than an order
of magnitude compared to a conventional 5 mm probe. This extreme
sensitivity jump makes the 1.7 mm Micro-CryoProbe an ideal tool for any NMR
analysis with limited sample amounts, e.g. natural products, isolated low
abundance proteins, peptides or small molecules, difficult-to-express
proteins, etc.
Conventional microprobes with 5 and 30 micro liter volumes have become very
popular in areas such as natural products chemistry, protein NMR and drug
screening applications. The introduction of a cryogenically cooled Micro-CryoProbe
is of significant importance for researchers working with very limited
sample quantities. These samples can be natural products isolated in minute
quantities from a variety of organisms or protein samples that have been
prepared in small scale expression systems or isolated from natural sources.
The combination of the gain achieved by cryogenic cooling both coil and
preamplifier and by proprietary high-sensitivity electronics designs leads to
this significant increase in sensitivity. For a given sample amount, a 6-fold
gain over a conventional 1.7mm probe and about 10-14 fold gain over a
conventional 5mm probe are obtained. This can lead to a 200-fold reduction in
experiment time, or alternatively it fundamentally enables NMR research with
very low sample quantities that previously would simply not have been possible
or practical.
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